提前获取早熟凝集染色体环快速估算受照剂量的可行性研究

doi:10.3969/j.issn.1004-616x.2011.06.010

›› 2011, Vol. 23 ›› Issue (6): 446-448.doi: 10.3969/j.issn.1004-616x.2011.06.010

提前获取早熟凝集染色体环快速估算受照剂量的可行性研究

汪传高，王治东，陈茂胜,杜杰,张学清,王仲文,陈英

1. 军事医学科学院放射与辐射医学研究所，北京100850；2. 中国原子能科学研究院，北京 102413

收稿日期:2011-09-19 修回日期:2011-09-22 出版日期:2011-11-30 发布日期:2011-11-30
通讯作者: 王治东

Feasibility of rapidly estimating biological dose under shortened incubation of premature chromosome condensation rings

WANG Chuan-gao1，2，WANG Zhi-dong，CHEN Mao-sheng，DU Jie，ZHANG Xue-qing，WANG Zhong-wen，CHEN Ying

1. Department of Radiation Toxicology and Oncology, Institute of Radiation Medicine, Beijing 100850; 2. China Institute of Atomic Energy, Beijing 102413, China

Received:2011-09-19 Revised:2011-09-22 Online:2011-11-30 Published:2011-11-30
Contact: WANG Zhi- dong

摘要/Abstract

摘要： 目的：缩短培养时间，拟合γ射线诱发离体人外周血淋巴细胞早熟凝集染色体环(premature condensation chromosome-rings，PCC-R)的剂量-效应曲线，探讨快速估算生物剂量的可行性。方法：60Co γ射线照射离体人外周血，剂量为0、1、3、6、10、15、20 Gy，照后分别培养44和48 h，收获前1 h加入冈田酸，分别计数PCC-R频率，拟合受照44与48 h的剂量-效应曲线并进行比较。结果：培养44 h较48 h获得的分析细胞数略少，PCC-R率略低于48 h结果(各个剂量点间的差异均无统计学意义)。外周血淋巴细胞PCC-R率随照射剂量的增加而增加，0~15 Gy剂量范围内二者之间满足直线方程Y = -0.015 2+0.062 5D(R2=0.983 9)。结论：缩短培养时间至44 h，PCC-R仍然可以用于15 Gy以内的辐射事故的剂量估算。

关键词: 大剂量辐射, 早熟凝集染色体环, 缩短培养时间, 剂量-效应曲线

Abstract: OBJECTIVE: To explore the feasibility of rapidly estimating biological dose under shortened incubation time and establish dose-effect curve of premature chromosome condensation rings (PCC-R) in human lymphocytes induced by γ-rays. METHODS： Human peripheral blood was irradiated in vitro by 60Co γ- rays at 0，1，3，6，10，15 and 20 Gy. PCC-R was counted after 44 h and 48 h incubation with Okadaic acid added 1 h before harvest. Both 44 h and 48 h dose-effect curves were established and compared. RESULTS：Cells obtained from 44 h incubation was slightly decreased，and PCC rings rate was slightly lowered compared with 48 h incubation ( P>0.05). PCC rings rate increased with dose and demonstrated a perfect linear relationship (0-15 Gy) ，with a linear equation Y= - 0.015 2+ 0.062 5D (R2=0.983 9). CONCLUSION：After shortening the incubation time to 44 h，PCC rings could still be used in dose estimation of high dose radiation incidents within 15 Gy.

Key words: high dose irradiation, premature chromosome condensation rings, shortened incubation time, dose-effect curves

汪传高，王治东，陈茂胜,杜杰,张学清,王仲文,陈英. 提前获取早熟凝集染色体环快速估算受照剂量的可行性研究[J]. , 2011, 23(6): 446-448.

WANG Chuan-gao1，2，WANG Zhi-dong，CHEN Mao-sheng，DU Jie，ZHANG Xue-qing，WANG Zhong-wen，CHEN Ying. Feasibility of rapidly estimating biological dose under shortened incubation of premature chromosome condensation rings[J]. , 2011, 23(6): 446-448.

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提前获取早熟凝集染色体环快速估算受照剂量的可行性研究

Feasibility of rapidly estimating biological dose under shortened incubation of premature chromosome condensation rings

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